Z-2177
ZytoLight® SPEC BCL6 Dual Color Break Apart Probe
green to orange, pkg of 0.2 mL, pkg of 0.05 mL
Synonym(s):
FISH, probe
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packaging
pkg of 0.05 mL, pkg of 0.2 mL, pkg of 20 tests, pkg of 5 tests
storage condition
protect from light
color
green to orange
application(s)
research use
storage temp.
2-8°C
General description
The ZytoLight® SPEC BCL6 Dual ColorBreak Apart Probe (PL136) is a probe for the qualitative detection oftranslocations involving the human BCL6gene at 3q27.3.
The fluorescence in situ hybridization (FISH) technique allows for the detection and visualization of specific nucleic acid sequences in cell preparations. Fluorescently-labeled DNA fragments, so called FISH probes, and their complementary target DNA strands in the preparations are codenatured and subsequently allowed to anneal during hybridization. Afterwards, unspecific and unbound probe fragments are removed by stringency washing steps. After counterstaining the DNA with DAPI, hybridized probe fragments are visualized using a fluorescence microscope equipped with excitation and emission filters specific for the fluorochromes with which the FISH probe fragments have been directly labeled.
ZytoLight SPEC BCL6 Dual Color Break Apart Probe is composed of:
- ZyGreen (excitation 503 nm/emission 528 nm) labeled polynucleotides (~10 ng/µl), which target sequences mapping in3q27.3* (chr3:186,737,897-187,403,834) proximal to the BCL6 breakpoint region
- ZyOrange (excitation 547 nm/emission 572 nm) labeled polynucleotides (~4.5 ng/µl), which target sequences mapping in3q27.3-q28* (chr3:187,744,962-188,097,195) distal to the BCL6 breakpoint region
- Formamide based hybridization buffer
Application
This probe is utilized in formalin-fixed, paraffin-embedded specimens, such as in the evaluation of B-cell lymphomas and for fluorescence in situ hybridization (FISH) techniques.
Analysis Note
- Hybridization signals of the probe appear green (proximal to the BCL6 breakpoint region) and orange (distal to the BCL6 breakpoint region)
- Normal situation: In interphases of normal cells or cells without atranslocation involving the BCL6 gene region, two green/orange fusion signals appear
- Aberrant situation: One BCL6 gene region affected by a translocation is indicated by one separate green signal and one separate orange signal
- Overlapping signals may appear as yellow signals
- Red blood cells present in the specimen might exhibit autofluorescence which hinders signal recognition
Legal Information
ZytoLight is a registered trademark of ZytoVision GmbH in Germany
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
Target Organs
Blood
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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