HPLC Analysis of Vitamins B1, B6 and B12 Using Ascentis® Express RP-Amide
Figure 1.HPLC Analysis of Vitamins B1, B6 and B12 using Ascentis® Express RP-Amide
Conditions | |
|---|---|
| Column | Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 5 µm particles (50773-U) |
| Column Temp. | 30 °C |
| Mobile Phase | [A] 0.02 M Ammonium Formate, pH = 3.0; [B] Acetonitrile |
| Gradient | 0-50% B in 1 min. Hold at 50% B until 1.5 min |
| Flow Rate | 1.5 mL/min |
| Pressure | 1639 psi (113 bar) |
| Sample | Dissolved in 50/50 water/ACN |
| Injection | 1 µL |
| Detector | UV, 276 nm |
Description | |
|---|---|
| Categories | Analytical Chromatography, Vitamins and Natural products |
| Featured Industry | Vitamins, Nutraceuticals, and Natural Products |
| Legal Information | Ascentis is a registered trademark of Sigma-Aldrich Co. LLC |
| Suitability | Application for HPLC |
Materials
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