04-281

Anti-phospho-EGFR (Thr669) Antibody, clone 5F10

clone 5F10, Upstate^®, from mouse

• Synonym(e): ERBB, ERBB1, epidermal growth factor receptor
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 5F10, monoclonal
• Species reactivity: human
• Application: WB
• Citations: 7

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5F10, monoclonal
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_005228.3, NM_201282.1, NM_201283.1, NM_201284.1
• UniProt accession no.: P00533
• shipped in: wet ice
• target post-translational modification: phosphorylation (pThr669)
• Gene Information: human ... EGFR(1956)

Beschreibung

General description

180 kDa

Immunogen

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 669 in human EGFR.

Application

Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Biochem/physiol Actions

Recognizes EGFR when phosphorylated at Threonine 669.

Physical form

100 µg of mouse monoclonal IgG1 liquid in 0.01 mg/ml PBS containing 0.09% sodium azide, PEG, and sucrose with 50 % glycerol.

Format: Purified

Thiophilic adsoption and size exclusion chromatography

Preparation Note

6 months at -20°C from date of shipment

Analysis Note

Control
Includes PMA and pervanadate treated A431 cell lysate as a positive control

Routinely evaluated by immunoblot.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids