05-384

Anti-α-Actinin Antibody, clone AT6/172

clone AT6/172, Upstate^®, from mouse

• Synonym(e): Anti-BDPLT15
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: AT6/172, monoclonal
• Application: ICC, WB
• Citations: 73

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: AT6/172, monoclonal
• species reactivity: rabbit, human, mouse
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_001102
• UniProt accession no.: P12814
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... ACTN1(87)

Beschreibung

General description

100 kDa

Immunogen

Human α-actinin

Application

Immunoprecipitation is not recommended

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-α-Actinin Antibody, clone AT6/172 is validated for use in IC, WB for the detection of α-Actinin.

Biochem/physiol Actions

Recognizes α-actinin.

Physical form

Format: Purified

Please see product datasheet for lot-specific formulation.

Protein A purified

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Routinely evaluated in immunoblot on human A431 cells

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable