06-288
Anti-Nck Antibody
Upstate®, from rabbit
Synonym(e):
Anti-NCK, Anti-nck-1
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Über diesen Artikel
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
23
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NCK1(4690)
General description
47kDa
Immunogen
Purified GST fusion protein produced in E. coli., corresponding to human Nck amino acid sequence 1 to 254
Application
Detect Nck using this Anti-Nck Antibody validated for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Biochem/physiol Actions
Nck
Physical form
0.1M Tris-glycine, pH 7.4, 0.15M NaCl containing 0.05% sodium azide
Format: Purified
Protein G Chromatography
Preparation Note
2 years at -20°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on human A431 carcinoma cells, mouse 3T3/A31 fibroblasts or rat L6 skeletal fibroblasts
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklasse
12 - Non Combustible Liquids
Was ist los?
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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