07-357

Anti-phospho-eNOS/NOS III (Ser114) Antibody

Upstate^®, from rabbit

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human, bovine
• Application: WB
• Citations: 3

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, bovine
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_000603
• UniProt accession no.: P29474
• shipped in: dry ice
• target post-translational modification: phosphorylation (pSer114)
• Gene Information: bovine ... Nos3(287024) ; human ... NOS3(4846)

Beschreibung

General description

132 kDa

Note: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS

Immunogen

Synthetic peptide corresponding to amino acids 106-118 of human eNOS/NOS III (Ser114) (PRKLQGRPpSPGPP-C) with a C-terminal cysteine added for conjugation to KLH. The immunizing sequence shares 12/13 amino acids with pig and bovine eNOS/NOS III.

Application

Detect phospho-eNOS/NOS III (Ser114) using this Anti-phospho-eNOS/NOS III (Ser114) Antibody validated for use in WB.

Research Category
Neuroscience

Research Sub Category
Oxidative Stress

Biochem/physiol Actions

eNOS/NOS III phosphorylated on serine 114

Physical form

0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA, and 0.05% sodium azide before the addition of glycerol to 30%

Immunoaffinity Chromatography

Preparation Note

1 year at -20°C

Analysis Note

routinely evaluated by immunoblot in fetal bovine heart endothelial cell lysate

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 1