07-889

Anti-phospho-PTEN (Ser370) Antibody

Upstate^®, from rabbit

• Synonym(e): MMAC1, TEP1, mutated in multiple advanced cancers 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: mouse
• Application: WB
• Citations: 9

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_000314.4
• UniProt accession no.: P60484
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer370)
• Gene Information: human ... PTEN(5728), TEP1(7011)

Beschreibung

General description

58 kDa

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-PTEN (Ser370)

Application

Anti-phospho-PTEN (Ser370) Antibody is an antibody against phospho-PTEN (Ser370) for use in WB.

Immunoblot Analysis: A 1:1000 dilution of this lot detected phospho-PTEN (Ser370) in RIPA lysates of 3T3-L1 cells.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Biochem/physiol Actions

Predicted to cross-react with human and rat based on 100 % sequence homology, but have not been tested.

Recognizes phosphorylated PTEN (Ser370)

Physical form

100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.

Affinity purified

Preparation Note

Stable for 2 years at -20°C from date of shipment.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Analysis Note

Routinely evaluated by immunoblot.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable