324676

Elastase, Pseudomonas aeruginosa

Elastase, Pseudomonas aeruginosa, is a native metallopeptidase that hydrolyzes insoluble elastin. Shares a significant sequence homology with thermolysin.

• Synonym(e): Pseudolysin
• EG-Nummer: 3.4.24.26 (BRENDA, IUBMB)
• NACRES: NA.77
• UNSPSC Code: 12352202
• Specific activity: ≥250 units/mg protein
• Assay: ≥90% (SDS-PAGE)

Eigenschaften

• Quality Segment: 200
• assay: ≥90% (SDS-PAGE)
• form: lyophilized
• specific activity: ≥250 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: aqueous buffer: 1 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Beschreibung

General description

Native elastase from Pseudomonas aeruginosa. A metallopeptidase that hydrolyzes insoluble elastin. Shares a significant sequence homology with thermolysin. Pseudomonas aeruginosa elastase has been implicated in the reinforcement of the inflammation process in cases of infection with the bacterium.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 2 months at -20°C.

Other Notes

Azghani, A.O., et al. 2000. Lung178, 181.
Kon, Y., et al. 1999. FEMS Immunol. Med. Microbiol. 25, 313.
Braun, P., et al. 1998. J. Bacteriol. 180, 3467.

One unit is defined as the amount of enzyme that will hydrolyze 1 µg insoluble elastin per h at 37°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable