401607

Goat Anti-Mouse IgG, H & L Chain β-Galactosidase Conjugate

liquid, Calbiochem^®

• NACRES: NA.46
• UNSPSC Code: 12352203
• Clone: polyclonal
• Species reactivity: mouse
• Application: —
• Citations: 2

Eigenschaften

• biological source: goat
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Enzyme Immunoassay (1:1000); Immunoelectrophoresis )
• isotype: IgG
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Beschreibung

General description

Immunoaffinity purified goat polyclonal antibody conjugated to β-galactosidase. Recognizes mouse IgG, heavy and light chains.

This Goat Anti-Mouse IgG, H & L Chain β-Galactosidase Conjugate is validated for use in Enzyme Immunoassay, Immunoelectrophoresis for the detection of Mouse IgG, H & L Chain.

Application

Enzyme Immunoassay (1:1000)

Immunoelectrophoresis (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM PBS, 10 mM glycine, 10 mM MgCl₂, 1% BSA, pH 7.0.

Other Notes

To achieve maximum enzyme activity, we recommend using the following substrate:

Dissolve 1 mg 4-methylumbelliferyl-β-D-galactoside (Cat. No. 474424) in 10 ml substrate buffer (10 mM sodium phosphate, pH 7.8, with 1 mM MgCl₂) by stirring for 30 min at room temperature. Remove undissolved substrate by centrifuging or filtration.



Monospecific for mouse IgG, heavy and light chains, as determined by immunoelectrophoresis against normal mouse serum. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• WGK: WGK 2