567693

SOST, Mouse, Recombinant

• NACRES: NA.41
• UNSPSC Code: 12352203
• Form: solid
• Assay: ≥90% (SDS-PAGE)

Eigenschaften

• assay: ≥90% (SDS-PAGE)
• Quality Segment: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: <1.0 EU/μg protein Endotoxin
• shipped in: ambient
• storage temp.: −20°C

Beschreibung

General description

Recombinant, mouse sclerostin (SOST) consisting of amino acids 24-211, expressed in NS0-derived murine myeloma cells with an N-terminal His•Tag. SOST is expressed by osteoclasts in developing bones of mouse embryos and acts as a negative regulator of bone formation through repression of BMP-induced osteogenesis.

Biochem/physiol Actions

Inhibition Assay: Inhibits hBMP-6-induced alkaline phosphatase by 50-80% in MC3T3-E1 mouse osteoblastic cells when used at a concentration of 30 µg/ml.

Physical form

Lyophilized from 0.2 µm filter-sterilized PBS, BSA.

Preparation Note

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 200 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Other Notes

Kusu, N. et al. 2003. J. Biol. Chem.278, 24113.
Balemans, W. et al. 2001. Hum. Mol. Genet.10 537.
Brunkow, M.E. et al. 2001. Am. J. Hum. Genet.68, 577.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3