This product is prepared at a concentration of 1 U/ul. A milligram concentration is not available. This RNase I solution also contains 10mM Hepes, pH 7.6, 10mM MgCl2, 10mM CaCl2, and 50% glycerol
69182
DNase I, RNase-frei
For applications in which maintenance of RNA integrity is critical
Synonym(e):
RNase-freie DNase I
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
Ansicht ändern
| Größe/SKU | Verfügbarkeit | Preis |
|---|
Über diesen Artikel
NACRES:
NA.85
UNSPSC Code:
12352202
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenform
liquid
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
technique(s)
DNA extraction: suitable
input
general use sample(s)
suitability
suitable for nucleic acid purification
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
General description
RNase-freie DNase I verdaut entweder einzel- oder doppelsträngige DNA, wobei eine Mischung aus Mono- und Oligonukleotiden entsteht. Dieses Präparat wurde so aufgereinigt, dass es frei von RNase ist, und eignet sich daher für Anwendungen, bei denen die Erhaltung der Integrität der RNA von entscheidender Bedeutung ist. Das Enzym baut in Gegenwart von RNA selektiv DNA ab und kann zur Entfernung von DNA-Templates nach In-vitro-Transkriptionsreaktionen verwendet werden. Dieses Enzym ist auch für andere Anwendungen wie DNase-Footprinting und Nick-Translation nützlich.
Other Notes
Eine Einheit baut 1 μg DNA in 10 Minuten bei 37 °C ab. Die Reaktionsmischung (50 µl) enthält 80 mM HEPES, pH 7,5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg Plasmid-DNA und Enzym.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxizität: Standard-Handhabung (A)
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description For applications in which maintenance of RNA integrity is critical | description - | description Novagen® | description Novagen′s PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. |
| technique(s) DNA extraction: suitable | technique(s) - | technique(s) - | technique(s) immunoblotting: suitable |
| form liquid | form liquid | form liquid | form liquid |
| suitability suitable for nucleic acid purification | suitability - | suitability - | suitability - |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) sample preparation |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
| storage condition avoid repeated freeze/thaw cycles, OK to freeze | storage condition OK to freeze | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze |
Lagerklasse
10 - Combustible liquids
Was ist los?
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
-
What is the concentration of this product in µg/mL?
1 answer-
Helpful?
-
-
Does DNase 1 (SKU 69182 and others) effectively work on circular DNA?
1 answer-
I came across a research article on ResearchGate that discusses DNase I and its effects on plasmid DNA. The article provides the following insights:
1) DNase I can degrade plasmid DNA, and its activity depends on the ionic strength of the reaction buffer. The enzyme exhibits optimal activity in a buffer containing Mg2+ and Ca2+. Micromolar levels of Ca2+ act as an enzyme activator in the presence of Mg2+. It is also mentioned that sample buffer interference (TE buffer), which chelates Ca2 and Mg2, might have affected the DNase treatment on pure plasmid.
2) The suggestion is to use a buffer containing Mg2+ and Ca2+, increase the concentration of DNase I, and incubate at 37°C for 10 minutes.
3) For circular DNA, the article recommends denaturing the circular DNA first, as DNase I degrades DNA by making random single-strand nicks in the phosphate backbone. When the DNA is circular, the fragments are likely to remain coiled together. To linearize the plasmid, two nicks should overlap, which is a rare event. The article suggests using denaturing agents such as urea to facilitate the unfolding of the plasmid or linearizing the plasmid with a specific primer before the DNase I treatment. It also questions the need to digest the plasmid to remove RNA, suggesting the use of an RNA-specific RNAse such as RNAse A.
Helpful?
-