The binding capacity of more than 85 nmol of free biotin per mL refers to 1 mL of settled agarose resin, not 1 mL of the 50% suspension. This product is supplied as a 50% slurry, meaning that 2 mL of suspension contains approximately 1 mL of settled resin. Therefore, 1 mL of suspension provides a binding capacity of approximately 42.5 nmol of free biotin or more.
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
5 mL | Warenkorb auf Verfügbarkeit prüfen | € 429,00 |
Über diesen Artikel
Quality Segment
form
liquid
manufacturer/tradename
Novagen®
storage condition
do not freeze
technique(s)
biotinylated protein purification: suitable
shipped in
ambient
storage temp.
2-8°C
General description
| Spezifikationen | |
| Konzentration | 50%ige Suspension (5 ml abgesetztes Volumen) |
| Speicherpuffer | 100 mmol/l Natriumphosphat pH-Wert 7,5; 0,02 % Natriumazid |
| Größe | 40165 µm |
| Bindungskapazität | > 85 nmol freies Biotin/ml |
| Protease | Nicht nachweisbar (SDS-PAGE von aufgereinigtem Protein) |
| DNase | Nicht nachweisbar (Kolonie-Assay blau/weiß von ligiertem Plasmid) |
| RNase | Nicht nachweisbar (Gel-Assay von aufgereinigter RNA) |
Legal Information
Disclaimer
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Dieser Artikel | |||
|---|---|---|---|
| description Column or batch isolation of biotinylated molecules | description suitable for affinity chromatography | description 5 mg Streptavidin for ELISA & Western Blotting. | description whole antiserum |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| form liquid | form suspension | form - | form - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. −20°C |
| storage condition do not freeze | storage condition - | storage condition - | storage condition - |
| manufacturer/tradename Novagen® | manufacturer/tradename - | manufacturer/tradename Chemicon® | manufacturer/tradename - |
| shipped in ambient | shipped in - | shipped in wet ice | shipped in dry ice |
Lagerklasse
12 - Non Combustible Liquids
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Does the binding capacity of > 85 nmol free biotin/ml refer to the 50% suspension or to the sedimented agarose?
1 answer-
Helpful?
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What is the recommended centrifuge speed for this product regarding biotinylated protein purification (especially washing steps)?
1 answer-
Centrifuge at 500 x g for 5 min.
Helpful?
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What is the total mass of the agarose in the 5ML pack size of product 69203-3, Streptavidin Agarose?
1 answer-
This product is provided as a 50% slurry, reflecting volume-to-volume ratio. The amount of streptavidin agarose used is strictly based on volume and not by its weight.
Helpful?
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