70922
BugBuster® HT Protein Extraction Reagent
Synonym(e):
Cell lysis reagent, Protein extraction buffer
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€ 251,00
€ 947,00
€ 1.690,00
€ 251,00
Über diesen Artikel
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Form
liquid
Qualitätsniveau
Hersteller/Markenname
Novagen®
Lagerbedingungen
OK to freeze
avoid repeated freeze/thaw cycles
Versandbedingung
wet ice
Lagertemp.
2-8°C
Verwandte Kategorien
1 of 4
Dieser Artikel | 70584-M | 70921 | 71186 |
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® |
| storage condition OK to freeze | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze |
| shipped in wet ice | shipped in - | shipped in ambient | shipped in wet ice |
| storage temp. 2-8°C | storage temp. 10-30°C | storage temp. 10-30°C | storage temp. - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level - |
Allgemeine Beschreibung
Anwendung
- to lyse the Escherichia coli cell pellets following centrifugation for protein expression and immunoblotting
- for protein extraction from bacterial cell pellet
- used to lyse the E. coli cell pellets for the expression and purification of dMIC60 (mitofilin) protein
Leistungsmerkmale und Vorteile
- Fast protein extraction and nucleic acid degradation
- Ideal for processing various samples of different volumes
- Increased extraction is ensured by the addition of rLysozyme solution
Rechtliche Hinweise
Haftungsausschluss
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Artikel
The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.
Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit
Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens
Verwandter Inhalt
Traditionally, protein purification from E. coli consists of four distinct phases: harvest, bacterial cell lysis, lysate clarification and protein purification. Bacterial lysis typically requires several time-consuming, hands-on steps, such as freeze/thaw cycles and sonication. These harsh lysis techniques may negatively impact protein quality and contribute to sample-to-sample variability. To maintain protein activity and integrity, detergent-based lysis buffers are routinely used to avoid mechanical protein extraction methods. Regardless of the lysis method used, centrifugation is traditionally required to pellet unwanted cell debris and permit recovery of the clarified lysate. The final step, purification, is frequently performed using affinity media specific for expressed epitope tags. Agarose-based media have typically been used, either as a slurry in microcentrifuge tubes or packed into gravity-driven or spin columns. While easier to manipulate, columns are greatly affected by lysate consistency and carryover of cell debris, which can lead to clogging of the column frits.
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