70923

BugBuster^® (primäraminfreies) Extraktionsreagenz

• Synonym(e): Protein Extraction Reagent
• NACRES: NA.77
• UNSPSC Code: 41106511

Eigenschaften

• form: liquid
• Quality Segment: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• input: bacteria and yeast sample(s)
• shipped in: ambient
• storage temp.: 10-30°C

Beschreibung

General description

Das BugBuster (primäraminfreie) Proteinextraktionsreagenz ist eine spezielle BugBuster Formulierung für Anwendungen, bei denen Primäramine im Proteinextrakt störend wirken würden, wie z. B. bei der Proteinimmobilisierung oder -vernetzung. Der in BugBuster (primäraminfrei) verwendete PIPPS-Puffer hat eine ähnliche Pufferkapazität und einen ähnlichen pH-Bereich wie der ursprüngliche Tris-gepufferte BugBuster, komplexiert aber keine Metallionen, wodurch er sich auch ideal für die Extraktion von metallabhängigen Proteinen eignet.

Features and Benefits

Primary Amine-Free Formulation: BugBuster^® (primary amine-free) Extraction Reagent is specially designed for applications where primary amines would interfere with protein extract. This means that academic researchers can purify metal-dependent proteins or proteins to be used for immobilization or crosslinking without worrying about the presence of primary amines, which could cause interference.

Enhanced Extraction: The extraction process can be further enhanced by the addition of rLysozyme Solution and Benzonase Nuclease, which helps to efficiently extract and purify proteins from the sample.

Metal Ion Compatibility: The PIPPS buffer used in the primary amine-free formulation of BugBuster^® has a similar buffer capacity and pH range as the original Tris-buffered BugBuster^® but will not complex metal ions. This makes it an ideal choice for extraction of metal-dependent proteins

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxizität: Standard-Handhabung (A)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 2