AB3121

Anti-Angiopoietin-2 Antibody, NT

Chemicon^®, from rabbit

• Synonym(e): Ang-2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA, WB
• Citations: 5

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, western blot: suitable
• NCBI accession no.: NM_001147.1
• UniProt accession no.: O15123
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Angpt2(11601)

Beschreibung

Immunogen

20 amino acid peptide sequence near the amino-terminus of mouse Ang-2.

Epitope: N-terminus

Application

Detect Angiopoietin-2 using this Anti-Angiopoietin-2 Antibody, N-terminus validated for use in ELISA & WB.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Western blot: 1-10 μg/mL using ECL. The predicted size of Ang-2 is ~55 kDa. Like Ang-1, its size may higher due to glycosylation.

ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide per well.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Recognizes mouse Angiopoietin-2 (Ang-2). Mouse/human Ang-2 is a 496 AA secreted angiogenic protein (Suri et al. 1996). There is ~85% homology between mouse and human Ang-2. It is only 60% homologous with Ang-1 (Davis et al. 1996). Ang-2 may act as an antagonist of Ang-1 and Tie-2 (Suri et al. 1996). It is prominently expressed in tissues that are in involved in vascular remodeling (ovary, placenta, and uterus).

The immunogenic peptide sequence is 71% (15/21 aa) homologous with human Ang-2 and has no substantial homology with Ang-1. Antibody crossreactivity in other species is not established. The immunogen peptide is 85% conserved in porcine and 55% in chicken.

Physical form

Affinity purified immunoglobulin presented as liquid in PBS containing 0.1% BSA and 0.05% azide, pH 7.5

Preparation Note

Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. May be stored at 2-8°C for short term use. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 2