ABC339

Anti-BFL-1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Bcl-2-related protein A1, Bcl-2-like protein 5, Bcl2-L-5, Hemopoietic-specific early response protein, Protein BFL-1, Protein GRS, BFL-1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Uniprot accession no.: Q16548
• Conjugate: unconjugated
• Application: IF, IHC, WB
• Citations: -

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse
• species reactivity (predicted by homology): human (immunogen homology)
• technique(s): immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
• UniProt accession no.: Q16548
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... BCL2A1(597)

Beschreibung

General description

Protein BFL-1, also known as protein GRS, Bcl-2-like protein 5 (Bcl2-L-5), and Bcl-2-related protein A1 (BCL2A1), is a member of the Bcl-2 family. BFL-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. BFL-1 is expressed in peripheral blood, spleen, bone marrow, lung, small intestine, and testis.

~39 kDa observed. Uniprot describes two isoforms at ~19 kDa and ~20 kDa

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human BFL-1.

Application

Anti-BFL-1 Antibody is a highly specific rabbit polyclonal antibody, that targets Bcl-2-related protein A1 & has been tested in western blotting, IHC & Immunofluorescence.

Immunohistochemistry Analysis: 2 µg/mL from a representative lot detected BFL-1 in mouse kidney tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected BFL-1 in mouse kidney cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Biochem/physiol Actions

This antibody recognizes the N-terminus of BFL-1.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse kidney tissue lysate

Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected BFL-1 in 15 µg of mouse kidney tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• wgk: WGK 2
• Lagerklasse: 12 - Non Combustible Liquids