ABE1069

Anti-Polyhomeotic-like protein 2 Antibody (PHC2)

from rabbit, purified by affinity chromatography

• Synonym(e): Polyhomeotic-like protein 2, hPH2, Early development regulatory protein 2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: -

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, rat, mouse
• technique(s): immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_932157
• UniProt accession no.: Q8IXK0
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PHC2(1912)

Beschreibung

General description

Polyhomeotic-like protein 2 (PHC2), also known as Polyhomeotic-like protein 2 hPH2, Early development regulatory protein 2, is encoded by the gene name PHC2, EDR2, and PH2. PHC2 is a component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class of proteins required to maintain the transcription repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex has been shown to function via chromatin remodeling and modification of histones. It also mediates monoubiquitination of histone H2A ′Lys-119′, rendering chromatin heritably changed in its expression. Polyhomeotic-like protein 2 PHC2 is a component of a Component of a PRC1-like complex. PHC2 has been demonstrated to interact with CBX4, BMI1, PCGF2, PHC1 and RNF2. In addition, PHC2 has been implied to interact with CHTOP and with the N-terminal region of the SP1 transcription factor and with MAPKAPK2.

~35/85 kDa observed. Uniprot describes 5 isoforms produced by alternative splicing at ~91 kDa, ~88 kDa, ~30 kDa, and ~36 kDa which are all expected to cross react to this protein.

Immunogen

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human Polyhomeotic-like protein 2 (PHC2) near the C-terminus.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Polyhomeotic-like protein 2 (PHC2) in human kidney tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-Polyhomeotic-like protein 2 antibody is validated for use in WB, IH for the detection of Polyhomeotic-like protein 2.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in rat kidney tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Polyhomeotic-like protein 2 (PHC2) in 10 µg of rat kidney tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1