CBA098

InnoCyte Monocyte Cell Migration Assay

• Synonym(e): Monocyte Migration Assay
• NACRES: NA.32
• UNSPSC Code: 12352207
• Technique(s): cell migration: suitable
• Usage: sufficient for 96 tests
• Storage temp.: no temp limit
• Detection method: fluorometric
• Shipped in: wet ice

Eigenschaften

• usage: sufficient for 96 tests
• Quality Segment: 100
• packaging: pkg of 1 96-well plate(s)
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• technique(s): cell migration: suitable
• input: sample type monocyte(s)
• detection method: fluorometric
• shipped in: wet ice
• storage temp.: no temp limit

Beschreibung

General description

The InnoCyte Monoctye Cell Migration Assay is designed to study the effects of various drugs or agents on monocyte motility or for identifying chemoattractant molecules. The assay is not intended for adherent cell lines, such as fibroblasts or solid tumor cell lines.

Biochem/physiol Actions

The Calbiochem InnoCyte 96-Well Monoctye Cell Migration Assay is intended for the study of the effects of various drugs or agents on monocyte motility or for identifying chemoattractant molecules. The assay is not intended for adherent cell lines, such as fibroblasts or solid tumor cell lines. To study these cell types, please use Cat. No. CBA010 or CBA017.

Preparation Note

Cell Labeling Mixture: Warm the D-PBS and Calcein-AM Solution to room temperature. Add 35 µl Calcein-AM Solution to 10 ml D-PBS. Mix well. Note: Prepare just before use (see step 7 of the Detailed Protocol below).

Upon arrival store the Calcein-AM at -20°C and the remaining components at 4°C.

Other Notes

96-Well Monocyte Cell Migration Chamber, Black, Conical 96-Well Plate, D-PBS, Calcein-AM, and a user protocol.

Cravens, P.D. 2007. Scan. J. Immunol.65, 514.
Charo, I.F., et al. 2006. N. Engl. J. Med.354, 610.
Knitscher, U., et al. 2000. Eur. J. Pharmacol.401, 259.
Turner, L., et al. 1999. Eur. J. Immunol.29, 2288.
Cross, A.K., et al. 1997. Cytokine9, 521.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Sicherheitshinweise

• Lagerklasse: 10-13 - German Storage Class 10 to 13
• Was ist los?: WGK 3