CP35

Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8)

liquid, clone 7E3-1B8, Calbiochem^®

• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: 7E3-1B8, monoclonal
• Species reactivity: rat
• Application: —
• Citations: 15

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 7E3-1B8, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: rat
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (Immunoblotting (1:2000); Immunofluorescence (1:200, indirect); Immunoprecipitation )
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Dlg4(29495)

Beschreibung

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with HL-1 mouse myeloma cells (see application references). Recognizes the ~95 kDa PSD-95 protein.

Recognizes the ~95 kDa PSD95 protein.

This Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of PSD-95 (Ab-2).

Immunogen

Rat

recombinant, rat PSD95

Application

Immunoblotting (1:2000)
Immunofluorescence (1:200, indirect)
Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Brakeman, P.R., et al. 1997. Nature386, 284.
Hualing, D., et al. 1997. Nature386, 279.
Kennedy, M.B. 1997. Trends in Neurosci.20, 264.
Sanes, J. 1997. Curr. Opin. Neurobiol.7, 93.
Cho., K.-O., et al. 1992. Neuron9, 929.

Immunofluorescent staining of rat hippocampal cells shows a staining pattern coincident with NMDA receptor staining at synaptic sites consistent with PSD-95′s proposed role in receptor clustering. This antibody has also been reported to work for immunoprecipitation. Fixation with cold methanol is recommended for immunofluorescence. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable