E5014

Benzonase ^® Salt Tolerant Endonuclease

≥200 U/uL, for nucleic acid removal at high salt concentration

• Synonym(e): Endonuclease, nuclease
• UNSPSC Code: 12352204
• NACRES: NA.21
• Form: buffered aqueous glycerol solution
• Biological source: Escherichia coli (recombinant, expressed in E.coli)
• Mol wt: calculated mol wt 27 kDa

Eigenschaften

• biological source: Escherichia coli (recombinant, expressed in E.coli)
• Quality Segment: 200
• form: buffered aqueous glycerol solution
• mol wt: calculated mol wt 27 kDa
• concentration: ≥200 units/μL
• input: general use sample(s)
• storage temp.: -10 to -25°C

Beschreibung

General description

The best-in-class solution for nucleic acid removal at high salt concentration. The Benzonase^® Salt Tolerant endonuclease is an enzyme, different in amino acids sequence from other Benzonase^® endonuclease references. It was designed with state-of-the-art protein engineering capabilities, to ensure the highest activity at high salt concentrations. The protein is a monomer with a molecular weight of about 27 kDa . The protein has an isoelectric point (pI) at pH 9.68. It is functional between pH 6 and 10, and from 0 °C to above 37 °C. Mg2+ (1–2 mM) is required for enzyme activity.

Application

Benzonase^® Salt Tolerant endonuclease degrades both DNA and RNA, whether single stranded, double-stranded, linear, circular or supercoiled. No base preference is observed. As with all endonucleases, Benzonase^® Salt Tolerant endonuclease hydrolyzes internal phosphodiester bonds present between the nucleotides. Upon complete digestion, all free nucleic acids present in solution are reduced to 5’-monophosphate terminated oligonucleotides, which are three to five bases in length.

Features and Benefits

• Active at higher salt concentration, up to1M NaCl
• Non-animal origin, recombinant from E.Coli, High Purity
• No post-translational modifications
• High batch to batch reproducibility
• Free of detectable protease activity
• Does not contain any antimicrobial preservatives or protein stabilizers except glycerol
• Compatible with Deviron^® detergents for cell lysis

Preparation Note

-10°C to -25°C

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

DEVIRON is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 1