GF320

CD40 Ligand, Human Recombinant
Animal Free

• Synonym(e): CD154, CD40 LIGAND, CD40L, GP39, hCD40L
• UNSPSC Code: 12352200
• NACRES: NA.75
• eCl@ss: 32160405

Eigenschaften

• impurities: <0.1 μg/mg protein endotoxin (<1 EU/μg)
• Quality Segment: 100
• UniProt accession no.: P29965
• shipped in: dry ice

Beschreibung

General description

CD40, a member of the TNF receptor superfamily, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to “immunodeficiency with hyper-IgM”, a disease characterized by failure to produce IgG, IgA and IgE.
Recombinant human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.

Product Source: Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.

Application

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

Physical form

Product is presented in 10mM Sodium Phosphate, pH 7.5 and is filtered through a 0.2 micron filter before lyophilization.

Preparation Note

Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.

Analysis Note

Bioactivity assay: Determined by its ability to stimulate the proliferation ofHEK-BlueTM CD40 reporter cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• WGK: WGK 1