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Merck

MAB8564

Anti-Poliovirus 3 Antibody, clone 613-F1-B5-E5

ascites fluid, clone 613-F1-B5-E5, Chemicon®

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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Produktname

Anti-Poliovirus 3 Antibody, clone 613-F1-B5-E5, ascites fluid, clone 613-F1-B5-E5, Chemicon®

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

613-F1-B5-E5, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable

isotype

IgG2a

shipped in

dry ice

Quality Level

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Dieser Artikel
MAB8560M7398SAB1403569
clone

613-F1-B5-E5, monoclonal

clone

583-G8-G2-A4, monoclonal

clone

BM-63, monoclonal

clone

6B3, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

technique(s)

immunofluorescence: suitable

technique(s)

immunofluorescence: suitable

technique(s)

indirect ELISA: 1:1000, indirect immunofluorescence: 1:500 using human PBL′s

technique(s)

indirect ELISA: suitable, western blot: 1:500-1:1000

Application

Anti-Poliovirus 3 Antibody, clone 613-F1-B5-E5 detects level of Poliovirus 3 & has been published & validated for use in IF.
IFA at 1:100-1:400.

Final working dilutions must be determined by end user.

SUGGESTED IFA

PROTOCOL:

Substrate slides:

1. Scrape and spot cells onto microscope slide wells.

2. Air dry.

3. Fix in cold acetone for 10 minutes at +4°C.

Stain:

1. Add 15 μL diluted polio antibody.

2. Incubate at +37°C for 30 minutes in a humid chamber.

3. Rinse in PBS for 5 minutes; repeat with fresh PBS.

4. Add 15 μL diluted labeled antibody.

5. Repeat steps 2 and 3.

6. Mount with buffered glycerol.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral

Biochem/physiol Actions

Reacts with the Poliovirus 3.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Store at -20°C for up to 12 months in convenient, undiluted aliquots. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklasse

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

William C Weldon et al.
Methods in molecular biology (Clifton, N.J.), 1387, 145-176 (2016-03-18)
Testing for neutralizing antibodies against polioviruses has been an established gold standard for assessing individual protection from disease, population immunity, vaccine efficacy studies, and other vaccine clinical trials. Detecting poliovirus specific IgM and IgA in sera and mucosal specimens has

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