MABC1641

Anti-Bcl-2 Antibody, clone 100

• Synonym(e): B-cell CLL/lymphoma 2 B-cell lymphoma protein 2
• UNSPSC Code: 12352203
• NACRES: NA.46
• Clone: 100, monoclonal
• Species reactivity: mouse, human
• Application: FACS, IHC, WB
• Citations: -

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 100, monoclonal
• form: liquid
• mol wt: calculated mol wt 26.27 kDa, observed mol wt ~26 kDa
• purified by: using protein G
• species reactivity: mouse, human
• species reactivity (predicted by homology): canine, feline
• packaging: antibody small pack of 100
• technique(s): flow cytometry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_000624
• UniProt accession no.: P10415
• storage temp.: 2-8°C
• Gene Information: human ... BCL2(596)

Beschreibung

General description

Apoptosis regulator Bcl-2 (UniProt: P10415; also known as Bcl2) is encoded by the BCL2 gene (Gene ID: 596) in human. Bcl-2 (B-cell lymphoma 2) is a pivotal anti-apoptotic protein belonging to the Bcl-2 family, which regulates cell survival and programmed cell death. Bcl-2 contains four Bcl-2 homology domains (BH1-BH4) and a transmembrane domain that anchors it to intracellular membranes, primarily the outer mitochondrial membrane. Its BH3 domain is critical for interactions with pro-apoptotic proteins such as Bax and Bak, inhibiting their activity and preventing mitochondrial outer membrane permeabilization and cytochrome c release. Bcl-2 is ubiquitously expressed across various tissues, with high levels in immune cells, epithelial cells, and neurons. Bcl-2 interacts with numerous proteins, including BH3-only proteins such as Bad and Bid, as well as non-Bcl-2 family proteins like Beclin-1, modulating autophagy and apoptosis pathways. Dysregulation of Bcl-2 expression is implicated in multiple diseases, including cancer, where its overexpression confers resistance to apoptosis and promotes tumor survival. Bcl-2 is also involved in neurodegenerative diseases like Alzheimer′s, where its imbalance contributes to neuronal death. Therapeutically, targeting Bcl-2 with BH3 mimetics has shown promise in treating hematological malignancies by restoring apoptotic sensitivity in cancer cells.

Immunogen

Thyroglobulin conjugated linear peptide corresponding to 14 amino acids from the N-terminal half of human Bcl-2.

Application

Quality Control Testing

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:500 Dilution from a representative lot detected Bcl-2 in Raji cell lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected Bcl-2 in Western Blotting application (Pezzella, F., et al. (1990). Am J Pathol. 137(2):225-32; Zhou, J., et al. (2019). Mol Cancer. 18(1):159).

Flow Cytometry Analysis: A representative lot detected Bcl-2 in Flow Cytometry application (Ludwig, L.M., et al. (2019). Methods Mol Biol. 1877:77-91).

Immunohistochemistry Applications: A representative lot detected Bcl-2 in Immunohistochemistry applications (Pezzella, F., et al. (1990). Am J Pathol. 137(2):225-32; Sciandra, M., et al. (2020). J Cell Commun Signal. 14(3):335-347).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Clone 100 is a mouse recombinant monoclonal antibody that detects Bcl-2. It targets an epitope within 14 amino acids from the N-terminal half.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable