MABD150

Anti-LIF Receptor, clone 1C7 (Azide Free) Antibody

clone 1C7, from mouse

• Synonym(e): Leukemia inhibitory factor receptor, LIF receptor, LIF-R, D-factor, CD118, GP190, LIFR
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 1C7, monoclonal
• Application: FACS, inhibition assay
• Citations: 5

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1C7, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable, inhibition assay: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001106857
• UniProt accession no.: P42703
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... LIFR(3977)

Beschreibung

General description

123 kDa calculated

The LIF receptor is critical in modern mouse stem cell maintenance as the receptor for LIF, the embryonic stem cell (ESC) growth factor that maintains pluripotency and self renewal in mouse ES cells, however physiologically, the importance of LIF-R in real mouse development has been less clear. LIF receptor does play a signaling role and when cleaved its extracellular portion can act as a decoy receptor and block exogenous LIF from performing its function on ES cells. LIF receptor is expressed in multiple tissues in the mouse during development from the liver to the lung and brain. LIF receptor also is important in immune responses and in the stimulation of the interferon response to viral infection. Also LIF receptor has recently been identified as a metastasis suppressor that functions through the Hippo-YAP pathway in humans.

Immunogen

Recombinant protein corresponding to mouse LIF Receptor.

Application

Anti-LIF Receptor, clone 1C7 (Azide Free) is an antibody against LIF Receptor for use in FC, Inhibition.

Inhibition Assay Analysis: A representative lot detected LIF Receptor in LIF binding to JAR cells (Blanchard, F., et al. (1997) International Immunology. 9(12):1775-1784).
Inhibition Assay Analysis: A representative lot detected LIF Receptor in IL3 proliferative response in Ba/F3 cells (Pitard, V., et al. (1997) Journal of Immunological Methods. 205:177-190).

Research Category
Stem Cell Research

Research Sub Category
Cell Cycle, DNA Replication & Repair

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Flow Cytometry in CHO D1IgD2-mycDAF cells .

Flow Cytometry Analysis: 1.0 µg of this antibody detected LIF Receptor in CHO D1IgD2-mycDAF.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 2