I am finding this same antibody from different sites and this specific one we used for western blot but didn't get the exact band segregation. even in the image here, it looks electronically modified. I tried it on a few cell lines but every time I got the same thicker bands around 30kD and almost nothing around 15-20kD.
MABE71
Anti-Histone Antibody, Pan, clone F152.C25.WJJ
clone F152C25W, from mouse
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Synonym(e):
Histone H1.4, Histone H1b
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 μg | Warenkorb auf Verfügbarkeit prüfen | € 545,00 |
Über diesen Artikel
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
F152C25W, monoclonal
Application:
ELISA, WB
Citations:
20
Technischer Dienst
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Unterstützung erhaltenbiological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
F152C25W, monoclonal
species reactivity
human, bovine
technique(s)
ELISA: suitable, western blot: suitable
isotype
IgG2aκ
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... H2AC21(317772)
General description
This antibody detects histone H1 and nuclesomal core proteins histones H2a, H2b, H3, H4. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Histone H1 is a nuclear protein necessary for condensation of nucleosome chains into higher order structures.
Histone H1 is a nuclear protein necessary for condensation of nucleosome chains into higher order structures.
~14-33 kDa observed
Application
Anti-Histone Antibody, Pan, clone F152.C25.WJJ is a Mouse Monoclonal Antibody for detection of Histone also known as Histone H1.4, Histone H1b & has been validated in WB, ELISA.
ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Mizoguchi, E., et al. (1997). Gastroenterology. 113:1828-1835.)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Physical form
Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Jurkat cell lysate
Jurkat cell lysate
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Histone H1 and core proteins on 10 µg of Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Histone H1 and core proteins on 10 µg of Jurkat cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB052
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Dieser Artikel | |||
|---|---|---|---|
| description clone F152C25W, from mouse | description from rabbit, purified by affinity chromatography | description clone 34, from mouse | description clone RM155, from rabbit |
| conjugate unconjugated | conjugate - | conjugate unconjugated | conjugate - |
| Gene Information human ... H2AC21(317772) | Gene Information human ... H2BC1(255626) | Gene Information human ... H1F0(3005) | Gene Information human ... H3F3B(3021) |
| biological source mouse | biological source rabbit | biological source mouse | biological source rabbit |
| clone F152C25W, monoclonal | clone polyclonal | clone 34, monoclonal | clone RM155, monoclonal |
| species reactivity human, bovine | species reactivity mouse, rat, human | species reactivity mouse, bovine, Xenopus, human, rat | species reactivity human |
| antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form purified antibody |
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