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MR-121

EmbryoMax® KSOM Mäuseembryo-Medien

(1X), Liquid, with 1/2 Amino Acids & Phenol Red

Synonym(e):

KSOM Media, K+ Simplex Optimised Medium (KSOM)

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Größe/SKUVerfügbarkeitPreis
50 mL
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€ 69,90

Über diesen Artikel

UNSPSC Code:
12352207
NACRES:
NA.75
eCl@ss:
32160801

€ 69,90


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Quality Segment

form

liquid

manufacturer/tradename

Specialty Media, EmbryoMax®

technique(s)

cell culture | embryo: suitable, cell culture | stem cell: suitable

input

sample type: mouse embryo(s)

Application

EmbryoMax® KSOM Mäuseembryo-Medien werden zur Sammlung von Mäuseembryos eingesetzt.

Preparation Note

Kann bei –20 °C bis zu dem auf der Flasche angegebenen Verfallsdatum gelagert werden.


Nach dem Auftauen sollte es innerhalb von 2 Wochen verwendet werden.

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Sofern in unserem Katalog oder anderen Begleitdokumenten unserer Produkte nicht anders angegeben, sind unsere Produkte nur für Forschungszwecke vorgesehen und nicht für andere Zwecke zu verwenden, einschließlich, jedoch nicht beschränkt auf unautorisierte kommerzielle Verwendung, zur In-vitro-Diagnostik, für Ex-vivo- oder In-vivo-Therapiezwecke oder jegliche Art der Einnahme oder Anwendung bei Menschen oder Tieren.


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Questions

1–4 of 4 Questions  
  1. Can KSOM (MR-121-D) be used to culture 2-PN mouse embryos to the blastocyst stage?

    1 answer
    1. As per this link below:
      EmbryoMax Advanced KSOM medium was shown to produce a higher frequency of blastocyst formation in multiple mouse strains after 72 hours in culture when compared with standard KSOM medium, including difficult to culture strains such as C57BL/6.
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/gene-expression-and-silencing/advanced-ksom-mouse-embryo-media?srsltid=AfmBOop5TYgU3q72NPBH0YDFF-aiKLreotmOFluo2Jaa2eLuR8CXwqY4

      EmbryoMax Advanced KSOM Medium, catalog number MR-101-D, can be used as a single medium to harvest and culture preimplantation mouse embryos, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture.
      https://www.sigmaaldrich.com/US/en/product/mm/mr101

      Helpful?

  2. Do I need to add antibiotic to it?

    1 answer
    1. MR-121 has Pen Strep in it.

      Helpful?

  3. HI, DOES IT HAVE ANY LACTATE IN THE MEDIUM? IF SO, WHAT CONCENTRATION?

    1 answer
    1. This product does contain Lactate. Please see the full product formulation below. Quantities are in mg/L.

      Helpful?

  4. Is the KSOM (MR-121-D) sufficient for embryo culture to the blastocyst stage on its own? In the literature, papers mention using "KSOM supplemented with amino acids." I observed that MR-121-D indicates it has "half amino acids." Essentially, I am trying to understand if individuals are adding extra amino acids to this media, or if the 1/2 amino acids already included are what they are referring to when they say "supplemented with amino acids."

    1 answer
    1. KSOM (MR-121-D) is indeed sufficient for embryo culture up to the blastocyst stage. It is a 1X liquid solution with 1/2 amino acids and Phenol Red. When scientists reference KSOM with 1/2 amino acids, that is the intended composition, and they do not add the remaining 1/2 amino acids.

      For more detailed information and historical context, the article "A brief history of the development of the KSOM family of media" provides insights into the development and usage of KSOM. According to documented literature and the mentioned article, KSOM has been shown to support a high yield of mouse blastocysts capable of developing into fetuses after transfer to surrogate mothers. The medium was notably improved when supplemented with 19 natural amino acids, with the added amino acids present at half the concentration described by Eagle. Dose response studies indicated little difference in the response between full strength and half strength concentrations of the amino acids.

      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790120/

      Helpful?

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