OP17

Anti-c-Fos (Ab-1) Mouse mAb (2G9C3)

liquid, clone 2G9C3, Calbiochem^®

• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: 2G9C3, monoclonal
• Species reactivity: rat, human, mouse
• Application: —
• Citations: 3

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2G9C3, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: rat, human, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Immunoblotting (2.5-5 µg/mL); Immunocytochemistry (2.5-5 µg/mL); Paraffin Sections (not recommended))
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... FOS(2353)

Beschreibung

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with AG.8653X mouse myeloma cells. Recognizes the ~55 kDa c-fos and ~62 kDa v-fos proteins.

Recognizes both the ~55 kDa c-Fos and ~62 kDa v-Fos proteins. Does not cross-react with the ~39 kDa Jun protein.

This Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of c-Fos (Ab-1).

Immunogen

Human

a synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos

Application

Immunoblotting (2.5-5 µg/ml)

Immunocytochemistry (2.5-5 µg/ml)

Paraffin Sections (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Positive Control
HeLa cells

Other Notes

Jensen, D.E., et al. 1991. Oncogene6, 1219.
Reiss, M., et al. 1990. Cancer Res.50, 6641.
RouscherIII, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.
Verma, I.M., et al. 1987. Cancer Res.49, 29.
Verma, I.M., et al. 1987. Cell51, 513.
Muller, R. 1986. Viochem. Biophys. Acta.823, 207.
Verma, P. 1986. Trends Genet.2, 93.
Greenberg, M., et al. 1984. Nature (London)311, 433.

Recognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• WGK: nwg