OP93

Anti-BRCA1 (Ab-2) Mouse mAb (MS13)

liquid, clone MS13, Calbiochem^®

• Synonym(e): Anti-Breast Cancer Susceptibility Protein
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: MS13, monoclonal
• Species reactivity: human
• Application: —
• Citations: 11

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: MS13, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Immunoblotting (2 µg/mL, chemiluminescence; ; Immunofluorescence ; Immunoprecipitation ; Paraffin Sections )
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... BRCA1(672)

Beschreibung

General description

Anti-BRCA1 (Ab-2), mouse monoclonal, clone MS13, recognizes ~220 kDa BRCA1 in MCF-7 cells. It is validated for Western blotting, immunofluorescence, immunoprecipitation, and for paraffin sections.

Purified mouse monoclonal antibody generated by immunizing mice with the specified iummunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~220 kDa BRCA1 protein.

Recognizes the ~220 kDa BRCA1 protein in MCF-7 cells. Does not react with blood group antigens or growth factor receptors such as EGFR. Sold under license of U.S. Patent 5,753,441 and 6,162,897.

Immunogen

Epitope: within amino acids 1-304

Human

recombinant, human BRCA1

Application

Immunoblotting (2 µg/ml, chemiluminescence; see application references)

Immunofluorescence (see application references)

Immunoprecipitation (see application references)

Paraffin Sections (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Positive Control
MCF7 cells

Other Notes

Antibody should be titrated for optimal results in individual systems.

Scully, R., et al. 1996. Science272, 123.
Gudas, J.M., et al. 1996. Cell Growth Differ.7, 717.
Vaughn, J.P., et al. 1996. Cell Growth Differ.7, 711.
Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet.11, 113.
Merajver, S.D., et al. 1995. Clin. Can. Res.1, 539.
Merajver, S.D., et al. 1995. Nat. Genet.9, 439.
Struewing, J.P., et al. 1995. Nat. Genet.11, 198.
Thompson, M.E., et al. 1995. Nat. Genet.9, 444.
Futreal, P.A., et al. 1994. Science266, 120.
Miki, Y., et al. 1994. Science266, 66.

Legal Information

Sold under license of U.S. Patents 5,753,441 and 6,162,897.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable