PC138

Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb

liquid, Calbiochem^®

• Synonym(e): Anti-Nuclear Factor ΚB
• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: polyclonal
• Species reactivity: human
• Application: —
• Citations: 4

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (ELISA ; Gel Shift ; Immunoblotting (1:500, chemiluminescence); Immunoprecipitation )
• isotype: IgG
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Beschreibung

General description

Rabbit polyclonal antibody. Recognizes the ~65 kDa NF-κB p65 (RelA) protein.

Recognizes the ~65 kDa NF-κB p65 (RelA) protein in Daudi cells.

This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2).

Immunogen

Human

a synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH

Application

ELISA (see comments)

Gel Shift (see comments)

Immunoblotting (1:500, chemiluminescence)

Immunoprecipitation (see comments)

Physical form

In 5 mM sodium phosphate buffer, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Daudi cells

Other Notes

Kolberg, R., 1997. J. NIH Research9, 29.
Van Antwerp, D.J. et al. 1996. Science274, 787.
Beg, A.A., et al. 1995. Nature376, 167.
Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141.
Wulczyn, F.G., et al. 1992. Nature358, 597.

This antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable