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NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
mouse, human
Application:
—
Citations:
17
Technischer Dienst
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Unterstützung erhaltenbiological source
rabbit
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1:3000-1:5000)
Immunofluorescence (1:300-1:500, indirect)
Immunoprecipitation (1:300-1:500))
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
target post-translational modification
unmodified
Gene Information
human ... TP53(7157)
General description
Purified rabbit polyclonal antibody. Recognizes the ~230 kDa 53BP1 protein.
Recognizes the ~230 kDa 53BP1 protein in HeLa cell nuclear extract (Cat. No. WB64).
This Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of p53 Binding Protein 1 (Ab-1).
Immunogen
Human
a recombinant protein consisting of human 53BP1 fused to a His•Tag sequence
Application
Immunoblotting (1:3000-1:5000)
Immunofluorescence (1:300-1:500, indirect)
Immunoprecipitation (1:300-1:500)
Immunofluorescence (1:300-1:500, indirect)
Immunoprecipitation (1:300-1:500)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM NaCl, 50 mM Tris-HCl, 20% glycerol, pH 7.6.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Analysis Note
Positive Control
HeLa Cell Nuclear Extract (Cat. No. WB64)
HeLa Cell Nuclear Extract (Cat. No. WB64)
Other Notes
Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Lagerklasse
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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