SCC048

N27 Rat Dopaminergic Neural Cell Line

Rat

• Synonym(e): Dopamine Neural Cells, 1RB3AN27 Cell Line
• UNSPSC Code: 41106514
• NACRES: NA.81
• eCl@ss: 32011203
• Biological source: rat

Eigenschaften

• Produktname: N27 Rat Dopaminergic Neural Cell Line, The N27 rat dopaminergic neural cell line has been used as a dopaminergic neuron model for in vitro and in vivo studies.
• biological source: rat
• Quality Segment: 100
• technique(s): cell culture | stem cell: suitable, cell culture: suitable using | mammalian
• shipped in: liquid nitrogen
• storage temp.: -140 to -196°C

Beschreibung

General description

Parkinson’s disease is a neurodegenerative disorder of the central nervous system that affects more than 6 million people worldwide. The motor symptoms of Parkinson′s disease result from the death of dopamine generating cells in the substantia nigra, a region of the midbrain. Numerous efforts have been made to slow the loss or replace these dopamine producing neurons in vivo.

The N27 rat dopaminergic neuron cell line was harvested from E12 rat mesencephalic tissue and was transfected with SV40 to immortalize the cell line. The N27 cell line, when injected into the striata of 6-hydroxydopamine-lesioned rats (an animal model of PD) caused a time-dependent improvement in neurological deficits. This immortalized cell line has been carefully characterized in studies of dopamine biosynthesis, neurotoxicity and used as a dopaminergic neuron model for in vitro and in vivo studies.

Application

Research Category
Stem Cell Research

Neuroscience

Research Sub Category
Neural Stem Cells

Neurochemistry & Neurotrophins

Neurodegenerative Diseases

Neuroregenerative Medicine

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

Biochem/physiol Actions

Neural Stem Cells

Neural Lineage Cells

Preparation Note

N27 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality.

Analysis Note

• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are negative for mycoplasma contamination.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable