SCC111
Synonym(e):
RSPO1-expressing 293T cells
| Größe/SKU | Verfügbarkeit | Preis |
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R-Spondin1-exprimierende 293T-Zelllinie, The R-Spondin1-Expressing 293T cell line produces RSPO1, a critical protein used to establish 3D organoid cultures from stomach, small intestine, colon, pancreas, and liver sources.
human
cell culture: suitable using | mammalian
dry ice
-140 to -196°C
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| description The R-Spondin1-Expressing 293T cell line produces RSPO1, a critical protein used to establish 3D organoid cultures from stomach, small intestine, colon, pancreas, and liver sources. | description recombinant, expressed in CHO cells, ≥95% (SDS-PAGE) | description NOTE: Both the cell line and DNA from the cell line may beavailable for this product. Please choose -1VL or VIAL for cells, or -DNA-5UGfor DNA, 12022001, human kidney, fetal. Small slightly rounded cells, dispersed growth | description affinity isolated antibody, buffered aqueous solution |
| technique(s) cell culture: suitable using | mammalian | technique(s) - | technique(s) cell culture: suitable using | mammalian | technique(s) immunohistochemistry: suitable, immunofluorescence: suitable, indirect ELISA: suitable, western blot: suitable |
| biological source human | biological source human | biological source human kidney (fetal) | biological source rabbit |
| shipped in dry ice | shipped in wet ice | shipped in dry ice | shipped in dry ice |
| storage temp. -140 to -196°C | storage temp. −20°C | storage temp. −196°C | storage temp. −20°C |
| Quality Level 100 | Quality Level - | Quality Level - | Quality Level 100 |
10 - Combustible liquids
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This product was stably transfected with a proprietary mammalian expression vector. The available datasheet confirms the construct but does not disclose the plasmid backbone.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/123/475/scc111dat-ms.pdf
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/422/301/scc111.pdf
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Organoids can be stained with antibodies for immunocytochemical (ICC)/Immunohistochemical (IHC) analysis using either whole-mount or paraffin embedding/sectioning techniques. It is critical to use antibodies prequalified for organoid analysis.
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Once isolated, organoids are cultured in specialized media embedded in a rich extracellular matrix (ECM) basement membrane extract hydrogel such as Growth Factor Reduced Matrigel. Media is replenished every other day and cells are passaged once per week (7-12 days) before cells become too large or necrotic. Cells can be passaged in either small clump fragments or as single cells using mechanical dissociation/enzyme-free passaging reagents. Depending on organoid type and confluency, split ratios of roughly 1:3-1:4 can be used when passaging organoids. For sensitive organoid types, ROCKi (Y27632) (SCM075) can be added during passaging to help in promoting cell viability.
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Traditional DNA/RNA isolation kits can be used to prepare genomic material for sequencing (RNA-seq, scRNA-seq, WGS, CHIP-seq etc). For some applications with large organoids, tissue specific DNA/RNA extraction kits are recommended. TRI Reagent (T9424) can be added to the dissociated organoids before RNA extraction and can be stored at -80 °C until future use.
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The cell line is selected by zeocin. It is not necessary to add zeocin to culture medium as the cell line is stable.
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