SCC229

YUMM 1.9 Mouse Melanoma Cell Line

• Synonym(e): Yale University Mouse Melanoma 1.9 cell line, Yale University Mouse Melanoma cell line
• UNSPSC Code: 41106514
• NACRES: NA.42
• eCl@ss: 32160703
• Biological source: mouse
• Manufacturer/tradename: Millipore

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• packaging: vial of ≥1 x 10^6 vial (viable cells per vial)
• manufacturer/tradename: Millipore
• technique(s): cell culture | mammalian: suitable
• shipped in: liquid nitrogen
• storage temp.: −196°C

Beschreibung

General description

Immunocompetent genetically-engineered mouse models harboring discrete genetic drivers of melanoma are essential for studying potential immunotherapies but are limited by the need to maintain colonies of multiple genotypes necessary to generate mouse models with appropriate genetic backgrounds.The YUMM (Yale University Mouse Melanoma) cell lines were developed as models that recapitulate genetic drivers found in a significant proportion of human melanomas.SourceYUMM1.9 mouse melanoma cells were derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57BL/6 mouse into which mutations from the Braf/Pten genetically-engineered mouse model had been introduced via backcrossing. References:1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.

Application

The YUMM1.9 cell line is driven by Braf activation, Pten inactivation, and Cdkn2a inactivation. The cell line is positive for the common melanoma marker, MelanA. The cells are syngeneic with C57BL/6J mice and are very tumorigenic in vivo.

Features and Benefits

​The YUMM cell models recapitulate genetic drivers found in a significant proportion of human melanomas. YUMM1.9 cells are driven by Braf activation, Pten inactivation, and Cdkn2a inactivation. The cell line is positive for the common melanoma marker, MelanA. The cells are syngeneic with C57BL/6J mice and are very tumorigenic in vivo.

Preparation Note

YUMM1.9 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable