SCC414

S462 Human Peripheral Nerve Sheath Tumor Cell Line

Human

• UNSPSC Code: 41106514
• NACRES: NA.28
• Biological source: human
• Growth mode: N/A
• Manufacturer/tradename: Sigma-Aldrich

Eigenschaften

• Produktname: S462 Human Peripheral Nerve Sheath Tumor Cell Line,
• biological source: human
• packaging: pkg of >1X10⁶ cells
• manufacturer/tradename: Sigma-Aldrich
• growth mode: N/A
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: ≤ − 140°C

Beschreibung

General description

Malignant peripheral nerve sheath tumors (MPNSTs) are soft tissue sarcomas that arise from peripheral nerves and result in high rates of local recurrence and hematogenous metastasis. Half of MPNSTs occur in patients with neurofibromatosis type 1 (NF1), a common autosomal dominant multisystem disorder caused by mutations of the NF1 tumor suppressor gene located on chromosome 17q. Loss of function of the intact NF1 allele, due to loss of heterozygosity (LOH) and somatic mutations of the gene, is associated with dermal and plexiform neurofibroma formation. Approximately 10% of NF1 patients develop MPNST, of whom only 21% survive for five years after diagnosis.

Application

Each vial contains ≥ 1X10⁶ viable cells.Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services Cells are negative for mycoplasma contamination.Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.

Malignant peripheral nerve sheath tumors (MPNSTs) are soft tissue sarcomas that arise from peripheral nerves and result in high rates of local recurrence and hematogenous metastasis. Half of MPNSTs occur in patients with neurofibromatosis type 1 (NF1), a common autosomal dominant multisystem disorder caused by mutations of the NF1 tumor suppressor gene located on chromosome 17q. Loss of function of the intact NF1 allele, due to loss of heterozygosity (LOH) and somatic mutations of the gene, is associated with dermal and plexiform neurofibroma formation. Approximately 10% of NF1 patients develop MPNST, of whom only 21% survive for five years after diagnosis. Source S462 is an established human MPNST cell line from a clinically and genetically well-characterized NF1 patient, with a nonsense germline mutation in exon 37 of the NF1 gene, LOH of the NF1 gene, LOH of the p53 gene, and a homozygous missense mutation in exon 4 of the p53gene.As a verified MPNST cell line, S462 is a valuable tool for the further investigation of the biology and pathogenesis of this malignancy as well as for in vitro pharmacologic studies essential for the development of new therapies.

Features and Benefits

S462 Human Peripheral Nerve Sheath Tumor Cell Line is a varified MPNST cell line and a valuable tool for the further investigation of the biology and pathogenesis of this malignancy.

Preparation Note

Cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Other Notes

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable