SCC593

3dGRO^® Endometrial Organoids (274T)

Serous Papillary Carcinoma, Endometrium, Age: 92, Female, Mutation: See Product Page

• Synonym(e): Endometrial Cancer Organoid, Endometrial Epithelial Organoid, Endometrial Tumor Organoid, Human Endometrial Organoids, Human Endometrial Tumor PDO
• UNSPSC Code: 41106514
• NACRES: NA.61

Eigenschaften

• Quality Segment: 100
• biological source: human endometrium
• packaging: vial of ≥1500 vial (viable cells per vial)
• manufacturer/tradename: Millipore
• gender: female
• technique(s): cell culture: suitable
• application(s): 3D cell culture
• control: (endometrium)
• relevant disease(s): carcinoma (serous papillary)
• shipped in: liquid nitrogen
• storage temp.: −196°C

Beschreibung

General description

Endometrial organoids are advanced three-dimensional cell culture systems derived from endometrial tissue, effectively mimicking the architecture and functionality of the uterus. These organoids serve as critical models for studying endometrial cancer, which includes various histological types with distinct biological behaviors and clinical outcomes.The predominant subtype, Endometrioid Adenocarcinoma, constitutes approximately 75-80% of endometrial cancer cases and is classified as Type I, often linked to estrogen exposure and diagnosed at earlier stages. In contrast, aggressive variants such as Serous Papillary Carcinoma (10-15%) and Clear Cell Carcinoma (5-10%) are classified as Type II endometrial cancers, frequently diagnosed at advanced stages and associated with poorer outcomes. Serous Papillary Carcinoma is particularly aggressive, characterized by high-grade features and mutations in the p53 gene, contributing to its malignancy and poor prognosis.The complexity of endometrial cancer is further illustrated by Mixed Endometrial Carcinoma, which contains multiple cancerous cell types, and Uterine Carcinosarcoma, which combines carcinoma and sarcoma components. Additionally, Undifferentiated Carcinoma, another Type II variant, presents unique challenges due to its aggressive nature. Rare variants, such as neuroendocrine tumors and lymphoepithelial-like carcinoma, further contribute to the diversity of endometrial cancers, emphasizing the need for accurate diagnosis and staging.We offer two lines of endometrial organoids, 274T and 291T, both derived from the serous type of endometrial cancer. The 274T organoid line is positive for PTEN, p53, MUC1, and Pan CD44 expression, while the 291T organoid line is positive for PTEN, MUC16 (CA-125), and MUC1. Both lines are negative for Estrogen Receptor (ER) and Progesterone Receptor (PR). These organoids enable researchers to investigate the unique properties of these aggressive cancer types, facilitating the exploration of personalized treatment options and enhancing our understanding and management of endometrial diseases.3dGRO^® organoids were derived utilizing HUB Organoid Technology. The purchaser of this product shall agree to HUB’s terms of use (listed below), which shall be separately acknowledged and accepted by such purchaser, prior to transfer of this product to purchaser. Cell Line Characteristics

• Sex: Female
• Age: 92 Years
• Organ: Endometrium (Primary Tumor)
• Disease: Serous Papillary Carcinoma

Application

• Viability: >1500 viable organoids/vial
• Organoid Growth: Pass
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Mycoplasma Contamination: Negative
• STR Profile: Pass

Features and Benefits

Cryopreserved patient derived endometrial cancer organoids (PDOs).

Preparation Note

Store in liquid nitrogen. The organoids can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Legal Information

3dGRO^® is a trademark of Merck KGaA, Darmstadt, Germany.
3dGRO^® organoids were derived utilizing HUB Organoid Technology.

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/pdouse. This product may not be re-engineered or copied; used to make derivatives or modifications; used to develop, test, or manufacture a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact organoids@milliporesigma.com.

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 2