ST1069

PhosphoDetect Anti-Paxillin (pSer¹⁷⁸) Rabbit pAb

liquid, Calbiochem^®

• UNSPSC Code: 12352203

Eigenschaften

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: mouse, human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• isotype: IgG
• storage temp.: 2-8°C

Beschreibung

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~68 kDa paxillin protein phosphorylated at Ser¹⁷⁸.

Paxillin is a focal adhesion adaptor protein associated with th establishment of focal adhesions, cell signaling via integrins, and cell motility. The N-terminus of paxillin contains a proline-rich region for SH3 and SH2 domain binding. The N-terminus also contains five copies of the LD motif for association of actin binding proteins. The C-terminus contains LIM domains and the focal adhesion targeting sequence. Paxillin is believed to function as an adaptor protein that facilitates assembly of multi-protein complexes and coordinates signaling via integrins. Tyrosine and serine phosphorylation of paxillin is associated with the establishment of cytoskeletal membrane attachments. Phosphorylation by JNK at Ser¹⁷⁸ is necessary for normal cell motility.

Recognizes the ~68 kDa paxillin protein phosphorylated at Ser¹⁷⁸.

This PhosphoDetect Anti-Paxillin (pSer¹⁷⁸) Rabbit pAb is validated for use in Immunoblotting for the detection of Paxillin (pSer¹⁷⁸).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁷⁸ phosphorylation site of human paxillin

Application

Immunoblotting (1:1000-1:5000)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In Tris-citrate/phosphate, pH 7.0-8.0.

Preparation Note

Following initial use aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cylces.

Analysis Note

Positive Control
NIH3T3 cells plated on fibronectin

Other Notes

Bellis, S.L., et al. 1997. Biochem. J.325, 375.
Schaller, M.D. 2001. Oncogene20, 6459.
Huang, C., et al. 2003. Nature424, 219.

Does not recognize unphosphorylated paxillin. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Highly Toxic (H)

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable