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NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
7D4, monoclonal
Species reactivity:
human
Application:
—
Citations:
-
Technischer Dienst
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Unterstützung erhaltenbiological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
7D4, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DPYD(1806)
General description
Anti-DPYD, mouse monoclonal, clone 7D4, recognizes the ~100 kDa DPYD protein in HeLa and HeLa NE cells. It is validated for use in ELISA, Western blotting, and immunocytochemistry.
Purified mouse monoclonal antibody. Recognizes the ~100 kDa DPYD protein.
Recognizes the ~100 kDa DPYD protein in HeLa and HeLa NE cells.
Immunogen
A recombinant polypeptide corresponding to amino acids of 1-111 human DPYD, expressed as a GST fusion protein
Application
ELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells, HeLa NE cells
HeLa cells, HeLa NE cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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Lagerklasse
12 - Non Combustible Liquids
WGK
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