DCTP-RO

2′-Desoxycytidin-5′-triphosphat Dinatriumsalz

99% (dNTP), <0.9% (dNDP), solution, 100 mM

• Synonym(e): dCTP-Na₂
• Empirische Formel (Hill-System): C₉H₁₄N₃Na₂O₁₃P₃
• CAS-Nummer: 102783-51-7
• Molekulargewicht: 511.12
• UNSPSC Code: 41106305
• PubChem Substance ID: 329798916
• MDL number: MFCD00084683
• NACRES: NA.54
• Assay: 99% (dNTP)
• Form: solution
• Storage temp.: −20°C

Eigenschaften

• description: PCR Grade, sodium salt
• assay: 99% (dNTP)
• form: solution
• usage: sufficient for 125,000 standard reactions (03732690001), sufficient for 31,250 standard reactions (11969021001), sufficient for 6,250 standard reactions (11934520001)
• packaging: pkg of 1,250 μL (11969021001 [100 mM]), pkg of 4 × 1,250 μL (03732690001 [4x100 mM]), pkg of 250 μL (11934520001 [100 mM])
• manufacturer/tradename: Roche
• concentration: <0.9% (dNDP), 100 mM
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2
• InChI: 1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1
• InChI key: ABWVCNMFYVEBIB-CDNBRZBRSA-L

Beschreibung

General description

dCTP, PCR Grade, is a 100 mM clear colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Application

2′-Desoxycytidin-5′-triphosphat-dinatriumsalz ist eines der Nukleotidtriphosphate, das für durch DNA-Polymerase ausgelöste Reaktionen wie z. B. Polymerase-Kettenreaktion, DNA-Sequenzierung und andere molekularbiologische Methoden verwendet wird.

Physical form

solution of dCTP in water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Analysis Note

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Other Notes

For general laboratory use.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash