POLYDIC-RO

Poly[d(I-C)]

lyophilized, pkg of 10 U (10108812001 [A₂₆₀ units]), pkg of 50 U (11219847001 [A₂₆₀ units])

• Synonym(e): Poly-deoxy-inosinic-deoxy-cytidylic acid
• UNSPSC Code: 12352200
• NACRES: NA.54
• Form: lyophilized
• Solubility: water: soluble
• Storage temp.: −20°C

Eigenschaften

• description: Poly-deoxy-inosinic-deoxy-cytidylic acid
• form: lyophilized
• mol wt: 28814 bp
• packaging: pkg of 10 U (10108812001 [A₂₆₀ units]), pkg of 50 U (11219847001 [A₂₆₀ units])
• manufacturer/tradename: Roche
• color: white
• mp: 54 °C (NaCl, 0.1 M, pH 7.0)
• solubility: water: soluble
• absorbance ratio: A_{290/260 nm} 0.24-0.32, A_{280/260 nm} 0.61-0.67, A_{250/260 nm} 1.18-1.30
• storage temp.: −20°C

Beschreibung

Application

Poly[d(I-C)] is used as a template for RNA polymerases, and as a DNA model for biophysical investigations.
It is also used as a primer template with Kornberg DNA polymerase for the determination of small amounts of dITP, dGTP, and dCTP. Poly(dI-dC) has been used in electrophoretic mobility shift assays (EMSA).[1]

Features and Benefits

Contents
Lyophilizate, sodium salt, base ratio 1:1, alternating base sequence

Preparation Note

Activator: Polymines (spermine, spermidine)
Storage conditions (working solution): Solution is stable to at least 12 months if stored at 2 to 8 °C, contact with DNase or bacteria is to avoid.

Analysis Note

Typical analysis:
Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀

Other Notes

1 A₂₆₀ unit corresponds to 50 μg ds DNA.

Unit Conversion: 1 A₂₆₀ unit corresponds to 50 μg ds DNA.

Chain Length approx. 20,000 bp

For life science research only. Not for use in diagnostic procedures.

In an aqueous solution the DNA itself is a salt (the acid groups of the phosphate back bone have a negative charge and are saturated by positive sodium ions) i.e. there is no additional salt in solution. The structural formula which shows the negative 0-groups in the phosphate backbone is available. The same number of positive sodium ions must be dissociated in solution as counter ions.

As the sodium ions are part of the product and saturate the phosphate groups, the lyophilisate is salt-free.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable