Yes, DMSO is a suitable solvent for this material. Stock solutions are typically prepared at 10 mM, however concentrations of up to 20 mg/mL have been achieved.
Other solvents include Dimethylformamide, Chloroform, Acetonitrile, and Methanol. Stock solutions may be store in aliquots at -20 °C. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/401/542/d7008pis.pdf
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D7008
Dihydroethidium
≥95% purity, powder
Synonym(e):
2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridin, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridin, Hydroethidin
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
10 mg | Warenkorb auf Verfügbarkeit prüfen | € 278,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C21H21N3
CAS-Nummer:
Molekulargewicht:
315.41
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Beilstein/REAXYS Number:
5107482
Technischer Dienst
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Unterstützung erhaltenProduktname
Dihydroethidium, ≥95%
Quality Segment
assay
≥95%
form
powder
technique(s)
titration: suitable
color
pink to very dark red
solubility
chloroform: 20 mg/mL
fluorescence
λex 370 nm; λem 420 nm (for cytoplasm of living cells), λex 535 nm; λem 610 nm (for chromatin of living cells)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
SMILES string
CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14
InChI
1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
InChI key
XYJODUBPWNZLML-UHFFFAOYSA-N
Application
Dihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC). It has been used to check the in situ generation of ROS (reactive oxygen species).
Redox-Indikator. Blaue Fluoreszenz vor der Oxidation zu Ethidium.
Packaging
abgefüllt unter Argon
Other Notes
B-Ring reduziertes Analogon von Ethidium
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description ≥95% | description BioReagent, suitable for fluorescence, ≥95% (HPCE) | description suitable for fluorescence, ~1% in H2O | description BioReagent, Molecular Biology, powder |
| technique(s) titration: suitable | technique(s) - | technique(s) - | technique(s) electrophoresis: suitable |
| color pink to very dark red | color - | color - | color - |
| fluorescence λex 535 nm; λem 610 nm (for chromatin of living cells), λex 370 nm; λem 420 nm (for cytoplasm of living cells) | fluorescence λex 392 nm; λem 410 nm in methanol, λex 358 nm; λem 461 nm | fluorescence λex 480 nm; λem 620 nm in H2O, λex 518 nm; λem 605 nm (bound to DNA), λex 530 nm; λem 600 nm in 50 mM phosphate buffer pH 7.0 (upon binding to DNA) | fluorescence λex 526 nm; λem 605 nm (10 mM TBE; pH 8.0;), λex 360 nm; λem 590 nm in PBS, λex 526 nm; λem 605 nm in aqueous buffer |
| form powder | form solid | form - | form powder |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) - |
| solubility chloroform: 20 mg/mL | solubility methanol: soluble, acetonitrile: soluble | solubility - | solubility - |
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Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
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Could DMSO be used as an alternative solvent?
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