UPRTBF

Ultra pure 10x Reverse Transcriptase Buffer

DNA-free, Nuclease-free 10x RT buffer, designed for further manufacture

Eigenschaften

• recombinant: expressed in E. coli expressed in
• Quality Segment: 300
• form: liquid
• feature: DNA free, RNase and DNase free (also Nickase free)
• packaging: pkg of 0.5 mL
• technique(s): RT-PCR: suitable, cDNA synthesis: suitable
• shipped in: dry ice
• storage temp.: -10 to -25°C

Beschreibung

General description

Ultra pure 10x Reverse Transcriptase Buffer is manufactured under stringent conditions to ensure that it is free of detectable levels of DNA, DNase, RNase, and Nickase to reduce the risk of false positive and false negative results in molecular diagnostic assays. Ultra pure 10x Reverse Transcriptase Buffer should be used with other ultra pure DNA-free reagents, such as Ultra pure High-temp Reverse Transcriptase and Ultra pure DNA-free Water, to minimize false positive and negative results and improve cDNA synthesis.
Ultra pure 10x Reverse Transcriptase Buffer was designed for use in highly-regulated industries in applications that require a high level of performance, lot-to-lot consistency, and high sensitivity without false results from reagent-borne contamination while meeting industry-driven critical regulations. To meet this requirements we have increased the quality level of our products. The M-Clarity Program defines product quality. It provides transparency so that you can choose, with confidence, suitable products that meet your needs with respect to quality and regulatory compliance, transparency, change notification, and documentation.

Application

Ultra pure 10x Reverse Transcriptase Buffer can be used for:

• Reverse transcription
• First strand cDNA synthesis (up to 7 kb)
• Preparation of cDNA libraries
• RT-PCR and RT-qPCR
• Rapid Amplification of cDNA Ends (RACE)
• RNA-Seq
• For further manufacture

Features and Benefits

• DNA-free
• DNase-free, RNase-free, and nickase-free
• Ideal for high throughput RT-PCR applications
• Compatible with Ultra pure High-temp Reverse Transcriptase
• Enhanced change control and quality agreement

Packaging

Ultra pure 10x Reverse Transcriptase Buffer is provided in larger SKU sizes for ease of use in high throughput RT-PCR applications and for further manufacture. Ultra pure 10x Reverse Transcriptase Buffer should be used with other ultra pure DNA-free reagents, such as Ultra pure High-temp Reverse Transcriptase and Ultra pure DNA-free Water, to minimize false positive and negative results.

Other Notes

Quality testing ensures the absence of detectable levels of contaminating DNA (<=1 copy of prokaryotic gDNA, <=0.1 copy of eukaryotic gDNA, and <=10 copies of plasmid DNA per 100 units of enzyme) as detected by qPCR using universal primers against 16S capable of detecting > 500,000 prokayotes, 18S capable of detecting > 110,000 eukaryotes, and Ori capable of detecting > 25,000 plasmids, respectively.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable