09278

4-[4-(Dimethylamino)phenylazo]benzoesäure-N-succinimidylester

≥98.0% (HPLC)

• Synonym(e): 4-(Dimethylamino)-azobenzol-4′-carbonsäure-N-succinimidylester, DABCYL-N-succinimidylester
• Empirische Formel (Hill-System): C₁₉H₁₈N₄O₄
• CAS-Nummer: 146998-31-4
• Molekulargewicht: 366.37
• UNSPSC Code: 12352108
• PubChem Substance ID: 329748752
• NACRES: NA.32
• MDL number: MFCD00467415

Eigenschaften

• assay: ≥98.0% (HPLC)
• form: powder
• solubility: DMF: soluble, DMSO: soluble
• fluorescence: λ_ex 428 nm; λ_em 453 nm
• storage temp.: −20°C
• SMILES string: CN(C)c1ccc(cc1)\N=N\c2ccc(cc2)C(=O)ON3C(=O)CCC3=O
• InChI: 1S/C19H18N4O4/c1-22(2)16-9-7-15(8-10-16)21-20-14-5-3-13(4-6-14)19(26)27-23-17(24)11-12-18(23)25/h3-10H,11-12H2,1-2H3/b21-20+
• InChI key: IBOVDNBDQHYNJI-QZQOTICOSA-N

Beschreibung

Application

A useful reagent (similar to MANCYL-SE) for labeling proteins or peptides through their amino-groups by forming stable peptide bonds. The succinimidyl ester is reactive with terminal amines or lysines of peptides and other nucleophiles for fluorescent studies of proteins. Its fluorescent properties (has a characteristic broad and intense visible absorption but has no fluorescence) make it an ideal long wavelength quencher and it has been utilized as an acceptor chromophore in FRET studies.

Analysis Note

Absorptionsspektrum zeigt ein Maximum bei 453 nm (in Methanol). Nach Reaktion mit Butylamin: max. Absorption bei 428 nm (Lit.)

Other Notes

N-terminale Modifikation von Peptiden in automatisierter Synthese.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)