10021101

SerBob cell line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA

• UNSPSC Code: 41106514
• Biological source: human prostate
• Relevant disease(s): cancer
• Growth mode: Adherent
• Morphology: Cobblestone
• Receptors: CD44, alpha2 integrin, alpha6 integrin, EGFR, FGFR1, LIFR
• Technique(s): cell culture | mammalian: suitable

Eigenschaften

• biological source: human prostate
• Quality Segment: 100
• form: liquid
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 10021101-DNA-5UG, pkg of vial of cells 10021101-1VL
• growth mode: Adherent
• karyotype: 4n (67 to 88)
• morphology: Cobblestone
• products: More differentiated than Bob with increased CD24, PAP, PSCA expression (prostate differentiation markers). 10% 3-D sphere formation in Matrigel. Migratory and invasive when compared to benign prostate tumour. More invasive than Bob.
• receptors: CD44, alpha2 integrin, alpha6 integrin, EGFR, FGFR1, LIFR
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Prostate cancer research including drug testing.

Biochem/physiol Actions

First spontaneously immortalized prostate cancer cell line to be established from a trans-rectal needle biopsy (TRBP) of a patient with castration-resistant prostate cancer (CRPC). Bob is a novel pre-clinical model for functional studies in CRPC and especially for studying the CRPC "basal" phenotype. SerBob grows as a monolayer culture with limited differentiation. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Human prostate cancer spontaneously immortalized

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,14
D16S539: 10,12
D5S818: 13
D7S820: 10,11
THO1: 9
TPOX: 8,10
vWA: 17

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Preparation Note

Keratinocyte-SFM (Invitrogen, catalogue no 37010-022, supplied with prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53), bovine pituitary extract (BPE) and glutamine) + 2ng/ml leukaemia inhibitory factor + 2ng/ml stem cell factor + 100ng/ml cholera toxin + 1ng/ml granulocyte macrophage colony stimulating factor.

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2-4 x 104 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. At confluence 5-7 x 104 cells/cm² can be expected.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable