14562

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluoreszeintetrakis(acetoxymethyl)ester

BioReagent, suitable for fluorescence

• Synonym(e): BCECF-AM
• Empirische Formel (Hill-System): C₃₉H₃₆O₁₉
• Molekulargewicht: 808.69
• UNSPSC Code: 12352108
• NACRES: NA.32
• MDL number: MFCD01862636

Eigenschaften

• product line: BioReagent
• Quality Segment: 100
• form: solid
• solubility: DMF: soluble, DMSO: soluble, acetonitrile: soluble
• fluorescence: λ_ex 482 nm; λ_em 528 nm in 0.1 M Tris pH 8.0 (esterase)
• suitability: suitable for fluorescence
• storage temp.: 2-8°C

Beschreibung

Application

An uncharged, nonfluorescent molecule that is useful for noninvasive bulk loading of cell suspension because it is capable of permeating the cell membrane. Cellular nonspecific esterases cleave its lipophilic blocking groups, resulting in a charged, fluorescent form of the molecule. Compared to the parent compound, this charged derivative leaks out of the cell at a much slower rate. Utilized for pH measurements in perfused tissues, intercellular spaces, mammalian cells, plant cells, bacteria, and yeast. Also, employed to investigate in assays for cellular functional properties such as adhesion, multi-drug resistance, viability and cytotoxicity, apoptosis and chemotaxis.

Cell-durchgängiger Ester von BCECF der, nach Hydrolyse durch zytosolische Esterasen, den im Zellinneren gefangenen pH-Indikator BCECF ergibt. Erlaubt die gleichzeitige Registrierung von Änderungen im Zellvolumen und des intrazellulären pH in einzelnen Osteosarkoma-Zellen

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)