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3014

Transcreener® GDP FI Assay

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NACRES:
NA.81
UNSPSC Code:
41122100

€ 399,00


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shipped in

dry ice

storage temp.

−20°C

General description

The Transcreener® GDP FI Assay is a competitive immunoassay for GDP with a red fluorescent intensity (FI) readout. Designed specifically for HTS and featuring a single addition mix-and-read format and room temperature reagent stability, the Transcreener® GDP FI Assay is compatible with plate readers typically found in research labs as well as those used in HTS core facilities. The 3014-A kit is formatted for 96-well plates while the 3014-1K and 3014- 10K kits are for 384-well and higher densities.

Able to accommodate GTP concentrations ranging from 0.1 to 1,000 μM, the Transcreener® GDP FI Assay provides excellent data quality (Z′ = 0.7) at low substrate conversion (typically 10% or less). Because the proprietary antibody used is highly selective for GDP, the assay can be used with any enzyme that converts GTP to GDP, including small G proteins. (Note that many commercially available GTPase preparations contain GDP as a stabilizer, which must be removed or diluted prior to use with this assay so as not to contribute to background signal.

View full Transcreener® product list

Physical form

Kit with buffered aqueous solutions

Preparation Note

3014-1K = 1,000 assay, 384-well
3014-10K = 10,000 assay, 384-well
3014-A = 200 assay, 96-well

Legal Information

Transcreener is a registered trademark of BellBrook Labs

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shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C




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Questions

  1. Could you provide references where the product has been used? We are planning to utilize this assay for measuring brain endosomal GTP.

    1 answer
    1. The following publication from BellBrook Labs may be of interest as a reference citation for this product: "Comparative Study J Virol. 2015 Nov;89(21):10879-90. doi: 10.1128/JVI.01744-15. Epub 2015 Aug 19." This study is titled "Functional Comparison of Mx1 from Two Different Mouse Species Reveals the Involvement of Loop L4 in the Antiviral Activity against Influenza A Viruses," and has the PMID: 26292322.

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