3018

Transcreener^® UDP² FP Assay

• Synonym(e): Transcreener assay
• NACRES: NA.84
• UNSPSC Code: 41122100
• Storage temp.: −20°C
• Shipped in: dry ice

Eigenschaften

• shipped in: dry ice
• storage temp.: −20°C

Beschreibung

General description

The Transcreener^® UDP2 FP Assay is a universal biochemical HTS assay for enzymes that produce UDP, including glycosyltransferase, galactosyltransferase, glucuronyltransferase, N-acetylglucosamyltransferase, N-acetlygalactosyltransferase, xylosyltransferase, and glycogen, cellulose, lactose and hyaluronan synthases. Enzyme activity is signaled by a decrease in fluorescence polarization as the bound tracer is displaced from the Transcreener^® UDP2 Antibody. The assay is a simple single step mix-and-read format enabling the use of unmodified native substrate concentrations of 1 – 1000 μM. The assay provides excellent signal at low substrate conversion, with a Z′ =0.7 and =100 millipolarization shift (mP) under normal reaction conditions.

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Physical form

Kit with buffered aqueous solutions

Preparation Note

3018-1K = 1,000 assay, 384-well
3018-10K = 10,000 assay, 384-well

Legal Information

Transcreener is a registered trademark of BellBrook Labs