37294

Dihydrofolatreduktase aus Rinderleber

~8 U/mL, ammonium sulfate suspension, off-white

• Synonym(e): Tetrahydrofolat-Dehydrogenase
• CAS-Nummer: 9002-03-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 1.5.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00130955
• Specific activity: ≥6 U/mg protein
• Biological source: bovine liver
• Concentration: ~8 U/mL

Eigenschaften

• biological source: bovine liver
• form: ammonium sulfate suspension
• specific activity: ≥6 U/mg protein
• concentration: ~8 U/mL
• color: off-white
• density: 1.2 g/mL at 20 °C
• storage temp.: 2-8°C

Beschreibung

Application

Dihydrofolate reductase (DHFR) is involved in the synthesis of purines, thymidine and glycine in folate metabolism. DHFR, from bovine liver, is used for methotrexate analysis . DHFR is useful to study various cancers such as sarcomas, leukemias and head and neck cancers .

Biochem/physiol Actions

Dihydrofolate reductase (DHFR) reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as an electron donor. The binding of antitumor agents, such as methotrexate, to DHFR prevents the formation of reduced folates, which are essential for DNA synthesis .

Physical form

Suspension in 3.6 M ammonium sulfate solution, pH 7.0

Other Notes

In folate metabolism for the synthesis of purines, thymidine and glycine

1 U entspricht der Enzymmenge, die 1 μmol 7,8-Dihydrofolat und NADPH zu 5,6,7,8-Tetrahydrofolat und NADP pro Minute bei pH 6.5 und 25°C umsetzt

Kann Verkaufsbeschränkungen unterliegen.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves