406-05A

Human Osteoblasts: HOb, adult

• Synonym(e): Hob cells
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human bone (normal)
• Growth mode: Adherent
• Morphology: osteoblast
• Relevant disease(s): arthritis; osteoporosis

Eigenschaften

• biological source: human bone (normal)
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: osteoblast
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): arthritis; osteoporosis
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

HOb are isolated from fetal or adult human bones. Additionally, osteoblasts from osteoarthritis or rheumatoid arthritis donors are available. Human osteoblasts provide an excellent model system for studies relevant to the skeletal system.

Characterization: Positive for bone mineralization.

Human osteoblasts have been used to:

• Characterize signaling pathways mediating bone morphogenesis, such as identify human Crossveinless-2 as an inhibitor of BMP (Binnerts, 2009); demonstrate crosstalk between Runx2, Osterix, and NELL-1 (Chen, 2011); identify TWEAK as a cytokine regulating RANTES production, BMP-2-induced differentiation, and RANKL expression in osteoblasts (Ando, 2006)
• Demonstrate that Kobophenol A enhances proliferation of osteoblasts via activation of the p38 pathway (Kwak, 2013) and clarify the relationship between hypertension and osteoporosis by demonstrating that Ang II activates osteoclasts via RANKL induction (Shimizu, 2008, 2012)
• Identify adiponectin as an activator of IL-6, IL-8, VEGF and MMPs in endothelial cells and osteoblasts, implicating it in the development of arthritis (Lee, 2014)
• Show that inhibition of NF-κB prevented the progression of bone loss in periodontitis and promoted the wound healing in bone defects through the inhibition of osteoclasts (Shimizu, 2009)
• Study active compounds from Uraria crinita for their potential to stimulate bone formation and regeneration (Mao, 2014)
• Investigate properties of osteosarcoma, including the role of fluid pressure in angiogenesis (Nathan, 2008) and novel oncogenes (Both, 2012); and develop better treatment strategies (Ma, 2011)
• Develop optimal materials, coatings and drug delivery techniques for orthopedic implants and tissue engineering (Frandsen, 2014; Ni, 2012; Pilia, 2013a, b, 2014; Shiels, 2012; Valente, 2012; Zhang, 2010, 2011)

Application

model for skeletal system research, signaling pathways, bone morphogenesis, gene expression, cell activation and proliferation, wound healing, bone formation and regeneration, treatment strategies, tissue engineering, in vitro bone models, mineralization, hormone treatments

Biochem/physiol Actions

Bone

Preparation Note

• 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 10 doublings

Please refer to the HOb Culture Protocol.

Other Notes

Basal Medium containing 10% FBS & 10% DMSO

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable