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51475

Hefe

lyticase substrate, powder

Synonym(e):

Dry brewer′s yeast

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Größe/SKUVerfügbarkeitPreis
500 g
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€ 27,50
2.5 kg
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€ 66,20

Über diesen Artikel

UNSPSC Code:
12352204
NACRES:
NA.83
MDL number:
MFCD00132595

€ 27,50

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Unterstützung erhalten

Produktname

Hefe, powder, slightly brown

form

powder

Quality Segment

color

slightly brown

application(s)

food and beverages

storage temp.

2-8°C

General description

Yeast is an inactive edible single-celled organism widely used in the food industry for baking, winemaking, and brewing. This product is dried irrespective of enzyme activity. Yeast serves as a substrate for lyticase.

Application

Yeast has been used:
  • as a structure modifier to alter the morphology and rheology of phase-separated biopolymer gels
  • to induce hyperthermia in rats to perform antipyretic activity test
  • in the extraction and enrichment method for the metaproteomic characterization of dissolved organic matter in groundwater samples

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Dieser Artikel
075339214489526
Sigma-Aldrich

Sigma-Aldrich

51475

Hefe

Hefeextrakt from Saccharomyces cerevisiae, powder, suitable for microbiology

Millipore

07533

Hefeextrakt

Hefeextrakt suitable for microbiology

Millipore

92144

Hefeextrakt

Hefeextrakt micro-agglomerated

Millipore

89526

Hefeextrakt

description

powder, slightly brown

description

from Saccharomyces cerevisiae, powder, suitable for microbiology

description

suitable for microbiology

description

micro-agglomerated

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

color

slightly brown

color

-

color

-

color

-

application(s)

food and beverages

application(s)

microbiology, food and beverages

application(s)

microbiology, food and beverages

application(s)

food and beverages
microbiology

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Lagerklasse

11 - Combustible Solids

Was ist los?

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

PSA (Persönliche Schutzausrüstung)

Eyeshields, Gloves, type N95 (US)

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Analysenzertifikate (COA)

Lot/Batch Number

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Questions

  1. I would like to know how to prepare the product for using it as a substrate in Lyticase activity assay: in what concentration, heat incubation? sonication?. I will appreciate any detailed information. Thank you

    1 answer

    1. The Sigma Enzymatic Assay for Lyticase utilizes Baker’s yeast as the substrate, following the protocol outlined below:
      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-lyticase
      While brewer’s yeast may be a suitable alternative, it is not guaranteed to exhibit identical behavior. Applying the same protocol is a reasonable starting point; however, experimental validation is strongly recommended to ensure reliable and reproducible results.

      Also, 1U of 04963 Sigma-Aldrich Lyticase from Bacillus subtilis corresponds to the amount of enzyme which decreases the absorbance at 800 nm by 0.001 per minute at pH 7.5 and 25 °C (Yeast, Cat. No. 51475, as substrate).
      https://www.sigmaaldrich.com/US/en/product/sigma/04963#product-documentation

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