53654

Anti-Kaninchen IgG−Abberior^® STAR 600 in Ziege hergestellte Antikörper

for STED application

• Synonym(e): Abberior^® STAR 600-Anti-Kaninchen IgG in Ziege hergestellte Antikörper
• NACRES: NA.45
• UNSPSC Code: 12352202
• Clone: polyclonal
• Species reactivity: rabbit
• Application: —
• Citations: 1

Eigenschaften

• biological source: goat
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: rabbit
• availability: available only in USA and Canada
• concentration: ~1 mg/mL
• fluorescence: λ_ex 604 nm; λ_em 623 nm in PBS, pH 7.4
• storage temp.: −20°C

Beschreibung

General description

Abberior STAR 600 was developed for STED and confocal microscopy in the orange spectral region. Abberior STAR 600 is highly photostable and bright orange fluorescent dye. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. It can be effectively excited with excitation light between 550 - 610 nm. Abberior STAR 600 can substitute dyes like Atto^™ 590, Atto^™ 594 or Alexa Fluor^™ 594. For STED microscopy, Abberior STAR 600 can be most efficiently used with a STED Laser wavelength between 750 - 800 nm. Abberior STAR 600 performs exceptionally well in combination with Abberior STAR 635P, STAR 635 or STAR RED. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λ_ex [nm]: 604 (PBS pH 7.4), 604 (H₂O), 601 (MeOH)
Extinction Coefficient, ε_max [M^-1cm^-1]: : 95 000 (PBS pH 7.4), 95 000 (H₂O), 100 000 (MeOH)
Correction Factor, CF₂₆₀ = ε₂₆₀/ε_max: 0,25 (PBS pH 7.4)
Correction Factor, CF₂₈₀ = ε₂₈₀/ε_max: 0,25 (PBS pH 7.4)
Fluorescence Maximum, λ_em [nm]: 623 (PBS pH 7.4), 623 (H₂O), 620 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,85 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,2 (PBS pH 7.4)

Application

Designed and tested for fluorescent super-resolution microscopy

Analysis Note

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Other Notes

Find more infomation here Abberior^®

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Atto is a trademark of Atto-Tec GmbH

abberior is a registered trademark of Abberior GmbH

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable